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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Blood Banking | 15–20% | - Blood group systems and immunology - Transfusion practice and testing - Blood products and components |
| Topic 2: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Topic 3: Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
| Topic 4: Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Topic 5: Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Topic 6: Laboratory Operations | 5–10% | - Instrumentation and laboratory mathematics - Quality assessment and safety |
| Topic 7: Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
If your reactions are strong at immediate spin (3+) and then get weaker at AHG (w+), it could mean the presence of a strong cold antibody.
Cold antibodies tend to be IgM and their optimum phase for reactivity is immediate spin. Incubation and washing of the sample may cause the agglutination that occurred at room temperature to break down. This would appear as a weaker reaction at AHG.
If the reaction strengths varied in each panel cell then that could be an indication that there are multiple antibodies present.
Your screen cells are 3+ at immediate spin and weak (W)+ at AHG. Your auto control is negative for both phases. Some of your antibody panel cells are 3+ at immediate spin and negative at AHG. What should you suspect?
A. A warm autoantibody is present
B. Bad specimen draw
C. A cold antibody may be present
Question 2
Angiotensin is an oligopeptide in the blood that causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenals.
A major action of angiotensin II is:
A. Increased vasoconstriction
B. Decreased adrenal secretion of aldosterone
C. Increased parathormone secretion by the parathyroid
D. Increased pituitary secretion of petressin
Question 3
Hemoglobin electrophoresis uses an electric field to separate hemoglobin molecules based on differences in net electrical charge. The rate of electrophoretic migration is also dependent on the ionic radius of the molecule, the viscosity of the solution through which it is migrating, the electrical field strength, temperature, and the type of supporting medium used.
Electrophoretic separation of hemoglobin fundamentally relies on:
A. Concentration differences of molecules
B. Shape variations of molecules
C. Net charge differences of molecules
D. Size differences of molecules
Question 4
Which of these advancements in the clinical laboratory has led to concerns about patient privacy
A. high test volumes
B. staffing with several levels of lab professionals
C. automated analyzers
D. computers
Question 5
A blood culture to evaluate septicemia is performed in:
A. urinalysis.
B. toxicology.
C. hematology.
D. microbiology.
Solutions:
| Question 1 Answer: C | Question 2 Answer: A | Question 3 Answer: C | Question 4 Answer: D | Question 5 Answer: D |


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